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KMID : 1041219960380010019
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Journal of Sericultural and Entomological Science
1996 Volume.38 No. 1 p.19 ~ p.24
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Isolation and Characterization of Inducible Genes from Bombyx mori Injected with E. coli by Differential Screening
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김상현/Kim, Sang Hyun
제연호/윤은영/강석우/김근영/강석권/Je, Yeon Ho/Yun, Eun Young/Kang, Seok Woo/Kim, Keun Young/Kang, Seok Kwon
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Abstract
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To investigate the genes which is related to immune reaction of Bombyx mori, differential screening was carried out using naive and induced B. mori mRNA probe. To begin with, we constructed the cDNA library with mRNA isolated from fifth instar larvae injected with E. coli(4 X 106 cells/larva) using Uni ZAP XR vector kit. Thirty-two inducible cDNAs showing higher intensity on the induced mRNA probing membranes were selected. Partial nucleotide sequences of 29 clones were determined and their expessed sequence tags (ESTs) were produced. Nineteen ESTs in 29 ESTs were matched in GenBank database and the rest of them were found to be unknown.. These unmatched ESTs were presumed to be novel genes. The nineteen ESTs contained variable genes related to biological process in Bombyx mori and four classes immune genes. four clones, BmInc 6, 8, 18 and 27 were similar to two antibacterial peptide genes, hemolin gene and transferrin gene, respectively.
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KEYWORD
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